4^th International Food Safety, Quality & Policy Conference

The genus Salvia L. (Lamicaeae) represented approximately 960 species all over the world while there are 99 species 55 of which are endemic in Flora of Turkey. Some species of the genus are consumed as herbal tea and some are used for traditional therapeutic purposes. Some members of the genus are an important export commodity. Dried herbal parts of S. virgata which were collected from Mersin, Turkey, 2014, were macerated with methanol at 25°C for 24 h. After evaporation of the methanol parts dry extract was used for LC-MS/MS analysis. Phenolic compound determination was performed using a Shimadzu 20A HPLC system coupled to an Applied Biosystems 3200 Q-Trap LC-MS/MS instrument equipped with an ESI ion source was used in the negative ionization mode. Separations were performed on a ODS 150 x 4.6 mm, i.d., 3 μm particle size, octadecyl silica gel analytical column operating at 40ºC at a flow rate of 1 mL/min. Aerial parts of S. virgata were hydrodistilled to obtain an essential oil that was then analyzed simultaneously by GC and GC/MS. Anticandidal and antibacterial effects of the oil and methanolic extract of aerial parts of S. virgata were evaluated against pathogenic Candida and bacteria strains by using CLSI M27-A2 and M7-A7 protocols respectively. Rosmarinic acid, was determined as the main compound of methanol extract while Caffeic acid, Luteolin glucoside were identified tentatively. Borneol (19.48 %), Camphor (14.64%), α-Pinene (8.9%) were found as major components of essential oil.