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Sheen Hee Kim

Sheen Hee Kim

Ministry of Food and Drug Safety, South Korea

Title: The method validation for Ochratoxin A in cereal-based foods by liquid chromatography

Biography

Biography: Sheen Hee Kim

Abstract

Ochratoxin A is a well-known nephrotoxic, hepatotoxic and carcinogenic mycotoxin, produced by several fungal species of the genera such as Penicillium spp. and Aspergillus spp. under various environmental conditions. The aim of this study is to optimize analytical method for determination of ochratoxin A in cereal-based foods. The chromatographic separation is performed by reversed phase HPLC with fluorescence detector. The method optimization is validated by extraction with solvent and clean-up on immune affinity column. The estimated recovery rates, at spiking levels 0.1 to 0.5 µg/kg, ranged from 94 to 114% with relative standard deviations <25%. This method show good sensitivity, accuracy and precision in cereal-based foods. The applicability of this method will be used in the chromatographic determination test of ochratoxin A to analyze cereal-based foods in Korea.